Crypt wendtii TC Study

Ben Belton · Post by **Ben Belton** » Sun Apr 05, 2009 4:48 pm

If you have the Crypt wendtii study that was given to us at the AGA in Atlanta, you might notice it leaves a lot out. I got up with Ghazanfar at Outback last night, and we reduced it to simple steps for your (my) ease of use.

MS = Murashige-Skoog

Step 1: Media for initial explant establishment
MS + 1mg/L of BAP + 30g of sugar/L
or
1/2 strength MS + 1mg/L of BAP + 30g of sugar/L

Step 2: Media for multiplication of explant tissue
MS + 4.5mg/L of BAP + 30g of sugar/L

Step 3: Media for growing out plantlets
MS + 0 BAP + 30g of sugar/L

Step 1 can be omitted, but it probably helps to get the explant started on a media with less sugar and BAP.

Check me on this G.

Ben

Ghazanfar Ghori · Post by **Ghazanfar Ghori** » Sun Apr 05, 2009 7:04 pm

Looks good.

Aaron · Post by **Aaron** » Sun Apr 05, 2009 9:00 pm

Step 2 can be repeated as many times as desired before going to step 3 correct?

Ben Belton · Post by **Ben Belton** » Sun Apr 05, 2009 9:04 pm

I believe that is correct. I think doing that is where you get zillions of plants to grow out.

Aaron · Post by **Aaron** » Sun Apr 05, 2009 9:11 pm

Ben Belton wrote:I believe that is correct. I think doing that is where you get zillions of plants to grow out.

Yes, that's where my greedy little plant mind was going with that question.

Ben Belton · Post by **Ben Belton** » Sun Apr 05, 2009 9:12 pm

The thought of 100,000 African Violates makes you crazy huh?

Aaron · Post by **Aaron** » Sun Apr 05, 2009 9:13 pm

Ben Belton wrote:The thought of 100,000 African Violates makes you crazy huh?

Ummm...yeah.

marydotson · Post by **marydotson** » Mon Apr 06, 2009 2:18 am

Catch me up on this...Is this what these formulas really mean:

Formula 1: You have one crypt and need to come up with a clean culture (cuz you never know what dirty stuff hangs out in swamps).
Formula 2: You have a clean culture growing--let's subculture it and re-subculture it until you have a zillion plantlets.
Formula 3: Crikey, your basement is filled with test tubes, but nothing growing is bigger than a grain of sand! Subculture a couple of those puppies into new flasks and get them to grow bigger!

Now I have to get more stuff growing immersed!

Ghazanfar Ghori · Post by **Ghazanfar Ghori** » Mon Apr 06, 2009 8:02 am

marydotson wrote:Catch me up on this...Is this what these formulas really mean:

Formula 1: You have one crypt and need to come up with a clean culture (cuz you never know what dirty stuff hangs out in swamps).
Formula 2: You have a clean culture growing--let's subculture it and re-subculture it until you have a zillion plantlets.
Formula 3: Crikey, your basement is filled with test tubes, but nothing growing is bigger than a grain of sand! Subculture a couple of those puppies into new flasks and get them to grow bigger!

You got it!

Ben Belton · Post by **Ben Belton** » Mon Apr 06, 2009 8:14 am

Step 2 from here forward shall be referred to as the "Greedy Aaron"

Crypt wendtii TC Study

Crypt wendtii TC Study

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