Logs

[Ghazanfar Ghori]

I've been getting better about keeping logs of all activity I'm doing with TC. With the amount of jars, media, mixes etc - it gets difficult very quickly to keep everything in memory. Small changes can affect the outcome and its no good to do TC and not be able to replicate/share accomplishments and failures.

They're simple - and designed to be that way otherwise I end up being
lazy about it.

Example Media log:

4/5/2009
Made 1 liter of media - 32 4oz jars ~30ml each
Product used: Caisson Labs MS Product 122 (Instead of logging everything thats in the mix)
1/2 strength MS

Color code: Blue

Added:
1 ml BAP (1 mg/l)
1 ml PPM
30 grams sugar
Adjusted pH to 5.7

Solidified with Agar (Phytotech) 6.5g

Batch code: M001 (You'll see where this comes in handy later)


Example Explant log:

4/6/2009
Initiated VFT (Venus Fly Trap) culture using flower stalks
Dipped in 70% alcohol for 5 seconds
Sterlized in 10% bleach for 20 minutes
Rinsed with sterile water for 10 minutes
Established 5 cultures on M001 media VFT01-05

..and I label each jar with with a code VFT01 - VFT05


Any observations can be logged using the jar code then.
Example:

5/10/2009
VFT01 culture is contaminated with fungus from the explant.
VFT02 culture has 5 shoots


Also, when transferring and explant to different media, you can carry the code though to be able to track something back all the way.

Example:

5/26/2009
VFT01 Transfered to shoot multiplication media - M002


If we all keep half decent logs, we'll have some information to share with others. Obviously, results may vary with each individual, but something is better than nothing.

[Ben Belton]

Which of the agars did you use? The 111 Phytotech agar is suppose to be stronger I have been told. Did you use 6.5g or 7.5?

[DelawareJim]

Nice simple log.

I'm assuming Batch M001 is the 1st batch you made up in March?

Cheers.
Jim

[Ghazanfar Ghori]

Which of the agars did you use? The 111 Phytotech agar is suppose to be stronger I have been told. Did you use 6.5g or 7.5?

The 'logs' above are just examples - not real data, though some of it might be. I'll share some real logs with you guys later tonight if you're interested.

[Ghazanfar Ghori]

Nice simple log.

I'm assuming Batch M001 is the 1st batch you made up in March?

Cheers.
Jim

Nah - this is just dummy data. And really, I've been making media for a 3 months now. Just started keeping logs in Feb.

[DelawareJim]

I guess you can take these last posts as a definite "Yes, please post the real logs."

Cheers.
Jim

[Ghazanfar Ghori]

I can post my logs - but use them at your own risk - since I don't know if the results are going to be positive yet.